Chambers first tried karting at age seven and began racing the following year at Oakland Valley Race Park in New York, winning the Kid Kart Championship in her debut season. She competed at club and regional level throughout the North East United States and Canada in Rotax Micro, Mini and Junior Max categories before stepping up to national events in X30 and KA100 Junior from 2018. Her standout karting result was a third-place finish in the X30 class at the 2019 SKUSA SuperNationals XXIII.
Chambers entered senior car racing in 2021 with a partial season in the Formula 4 United States Championship for Future Star Racing, scoring a single point with a tenth-place finish at Mid-Ohio and ending the season 26th overall. She tested in a one-day FIA Formula 3 session at Circuit de Nevers Magny-Cours in September 2022, completing 50 laps.
Following a W Series pre-season test in Arizona and Barcelona in early 2022, Chambers was confirmed for the 2022 W Series season with Jenner Racing alongside Jamie Chadwick. She earned points in the Miami round with tenth place as the highest-placed rookie, but gathered just one championship point across the season and finished 16th in the standings. Also in 2023, she competed in the Formula Regional Oceania Championship with Giles Motorsport, finishing ninth in the standings and earning the Most Improved Driver award. Notably she secured pole position and victory in the second race at Taupo, becoming the first woman to start from pole and win in series history.
In 2023 Chambers joined the Porsche Sprint Challenge North America as a Porsche Junior after being named to the Porsche Deluxe Female Driver Development Program. Across a partial season she won eight races and stood on the podium nine times from 12 starts, finishing sixth in the overall standings. She claimed pole and back-to-back wins in her first weekend at Barber Motorsports Park and added a further victory at Virginia International Raceway. She returned in 2024 as a guest driver at Barber, again taking pole and winning both races.
Chambers joined F1 Academy for the 2024 season with Campos Racing, supported by Haas. She scored her first podium in Race 1 at Miami, passing Hamda Al Qubaisi to take third. In Barcelona she qualified on the front row for both races; after finishing third in Race 1, she overtook pole-sitter Abbi Pulling at the start of Race 2 and led throughout to claim her maiden F1 Academy win, finishing over six seconds clear. Chambers collected one win and four podiums over the season to place sixth in the championship on 122 points.
Retained by Campos Racing for 2025, Chambers moved into the Red Bull Ford Academy Programme. She won Race 3 of Round 4 in Canada from pole and claimed her second victory in Race 2 of the season finale in Las Vegas, leading every lap and setting fastest lap at her home event. She finished third in the championship standings with 127 points, behind Doriane Pin on 172 and Maya Weug on 157.
Chambers tested in Formula E machinery at the November 2024 women's test with Andretti at Jarama. At the 2025–26 pre-season women's test in Valencia she drove for Mahindra Racing, topping both test sessions with the fastest female time of 1:22.767. She also participated in the rookie free practice session for Mahindra at the Miami ePrix. For 2026 she is set to race in the Lamborghini Super Trofeo North America for RAFA Racing Club, supported by Monoflo International.
Chambers was adopted from China at eleven months old by Matthew and Shannon Chambers. Her sister Emma is also adopted from China and her brother Oliver from Ethiopia; all three siblings are adopted. She is an ambassador for the non-profit Gift of Adoption Fund. On 21 August 2020 she set the Guinness World Record for the fastest vehicle slalom, navigating 50 cones in 47.45 seconds in a 2020 Porsche 718 Spyder.